A New Approach to Supramolecular Structure Determination in Pharmaceutical Preparation of Self-Assembling Peptides: A Case Study of Lanreotide Autogel.
Manuela GrimaldiAngelo SantoroMichela BuonocoreClaudio CrivaroNicola FunicelloMatilde Sublimi SaponettiCristina RipoliManuela RodriquezSalvatore De PasqualeFabrizio BobbaLucia FerrazzanoWalter CabriAnna Maria D'UrsiAntonio RicciPublished in: Pharmaceutics (2022)
The supramolecular structure in peptides' prolonged-released gel formulations is the most critical parameter for the determination of the pharmaceutical profile of the drug. Here, we report our investigation on lanreotide Autogel as a case study. For the first time, we describe the use of the pulsed field gradient (PFG) diffusion-ordered spectroscopy (DOSY) magic-angle spinning NMR to characterize the supramolecular self-assembly and molecular mobility of different samples of lanreotide Autogel formulations prepared according to different formulation protocols. The diffusion coefficient was used to calculate the hydrodynamic radii of supramolecular assemblies and build relative molecular models. DOSY data were integrated with NMR imaging (MRI) measurements and atomic force microscopy (AFM) imaging.
Keyphrases
- high resolution
- atomic force microscopy
- high speed
- solid state
- single molecule
- energy transfer
- water soluble
- molecularly imprinted
- magnetic resonance
- solid phase extraction
- mass spectrometry
- magnetic resonance imaging
- diffusion weighted imaging
- electronic health record
- tandem mass spectrometry
- big data
- amino acid
- contrast enhanced
- emergency department
- quantum dots
- deep learning
- computed tomography
- machine learning
- fluorescence imaging
- data analysis