Self-Assembled Cationic Polypeptide Supramolecular Nanogels for Intracellular DNA Delivery.
Sharafudheen Pottanam ChaliSabine HüwelAndrea RentmeisterBart Jan RavooPublished in: Chemistry (Weinheim an der Bergstrasse, Germany) (2021)
Supramolecular nanogels are an emerging class of polymer nanocarriers for intracellular delivery, due to their straightforward preparation, biocompatibility, and capability to spontaneously encapsulate biologically active components such as DNA. A completely biodegradable three-component cationic supramolecular nanogel was designed exploiting the multivalent host-guest interaction of cyclodextrin and adamantane attached to a polypeptide backbone. While cyclodextrin was conjugated to linear poly-L-lysine, adamantane was grafted to linear as well as star shaped poly-L-lysine. Size control of nanogels was obtained with the increase in the length of the host and guest polymer. Moreover, smaller nanogels were obtained using the star shaped polymers because of the compact nature of star polymers compared to linear polymers. Nanogels were loaded with anionic model cargoes, pyranine and carboxyfluorescein, and their enzyme responsive release was studied using protease trypsin. Confocal microscopy revealed successful transfection of mammalian HeLa cells and intracellular release of pyranine and plasmid DNA, as quantified using a luciferase assay, showing that supramolecular polypeptide nanogels have significant potential in gene therapy applications.
Keyphrases
- water soluble
- drug delivery
- circulating tumor
- gene therapy
- cell free
- single molecule
- cancer therapy
- energy transfer
- cell cycle arrest
- reactive oxygen species
- escherichia coli
- induced apoptosis
- ionic liquid
- high throughput
- photodynamic therapy
- oxidative stress
- mass spectrometry
- cell death
- molecularly imprinted
- amino acid
- high resolution
- cell proliferation
- circulating tumor cells
- solid phase extraction
- quantum dots