Enhanced Catalytic Activity of a New Nanobiocatalytic System Formed by the Adsorption of Cytochrome c on Pluronic Triblock Copolymer Stabilized MoS 2 Nanosheets.

The formation of nanobiohybrids through the immobilization of enzymes on functional nanomaterials has opened up exciting research opportunities at the nanobiointerfaces. These systems hold great promise for a wide range of applications in biosensing, biocatalytic, and biomedical fields. Here, we report the formation of a hybrid nanobiocatalytic system through the adsorption of cytochrome c (Cyt c ) on pluronic triblock copolymer, P123 (PEO- b -PPO- b -PEO), stabilized MoS 2 nanosheets. The use of pluronic polymer has helped not only to greatly stabilize the exfoliated MoS 2 nanosheets but also to allow easy adsorption of Cyt c on the nanosheets without major structural changes due to its excellent biocompatibility and soft protein-binding property. By comparing the catalytic activity of the Cyt c -MoS 2 nanobiohybrid with that of the free Cyt c and as-prepared MoS 2 nanosheets, we have demonstrated the active role of the nanobiointeractions in enhancing the catalytic activity of the hybrid. Slight structural perturbation at the active site of the Cyt c upon adsorption on MoS 2 has primarily facilitated the peroxidase activity of the Cyt c . As the MoS 2 nanosheets and the native Cyt c individually exhibit weaker intrinsic peroxidase activities, their mutual modulation at the nanobiointerface has made the Cyt c -MoS 2 a novel nanobiocatalyst with superior activity.