Upconversion nanoscopy revealing surface heterogeneity of tumor-secreted extracellular vesicles.

Published in: Light, science & applications (2023)

Super-resolution microscopic imaging employing upconversion nanoparticles is applied to reveal the surface heterogeneity of tumor cell-derived small extracellular vesicles, i.e., exosome. The number of surface antigens of every extracellular vesicles can be quantified by both the high imaging resolution and stable brightness of upconversion nanoparticles. This method proves its great potential in nanoscale biological studies.

Keyphrases

photodynamic therapy
single cell
high resolution
energy transfer
fluorescence imaging
dendritic cells
gene expression
immune response
climate change
case control
walled carbon nanotubes