Phagocytosis of a PFOB-Nanoemulsion for 19F Magnetic Resonance Imaging: First Results in Monocytes of Patients with Stable Coronary Artery Disease and ST-Elevation Myocardial Infarction.
Fabian NienhausDenise ColleyAnnika JahnSusanne PfeilerVera FlockeSebastian TemmeMalte KelmNorbert GerdesUlrich FlögelFlorian BönnerPublished in: Molecules (Basel, Switzerland) (2019)
Fluorine-19 magnetic resonance imaging (19F MRI) with intravenously applied perfluorooctyl bromide-nanoemulsions (PFOB-NE) has proven its feasibility to visualize inflammatory processes in experimental disease models. This approach is based on the properties of monocytes/macrophages to ingest PFOB-NE particles enabling specific cell tracking in vivo. However, information on safety (cellular function and viability), mechanism of ingestion and impact of specific disease environment on PFOB-NE uptake is lacking. This information is, however, crucial for the interpretation of 19F MRI signals and a possible translation to clinical application. To address these issues, whole blood samples were collected from patients with acute ST-elevation myocardial infarction (STEMI), stable coronary artery disease (SCAD) and healthy volunteers. Samples were exposed to fluorescently-labeled PFOB-NE and particle uptake, cell viability and migration activity was evaluated by flow cytometry and MRI. We were able to show that PFOB-NE is ingested by human monocytes in a time- and subset-dependent manner via active phagocytosis. Monocyte function (migration, phagocytosis) and viability was maintained after PFOB-NE uptake. Monocytes of STEMI and SCAD patients did not differ in their maximal PFOB-NE uptake compared to healthy controls. In sum, our study provides further evidence for a safe translation of PFOB-NE for imaging purposes in humans.
Keyphrases
- magnetic resonance
- st elevation myocardial infarction
- contrast enhanced
- percutaneous coronary intervention
- magnetic resonance imaging
- coronary artery disease
- st segment elevation myocardial infarction
- dendritic cells
- diffusion weighted imaging
- peripheral blood
- coronary artery bypass grafting
- acute coronary syndrome
- flow cytometry
- computed tomography
- cardiovascular events
- health information
- oxidative stress
- high resolution
- mass spectrometry
- cardiovascular disease
- healthcare
- social media
- immune response
- heart failure
- positron emission tomography
- cell therapy
- prognostic factors
- pet imaging
- photodynamic therapy
- fluorescence imaging