An automatic whole blood analyzer for renal function analysis with a centrifugal microfluidic device.
Chuang WangYusheng FuJiuchuan GuoJin Hong GuoPublished in: The Analyst (2022)
In recent years, chronic kidney disease (CKD) has received widespread attention as one of the fastest growing non-communicable diseases (NCD) worldwide. Here, a clinical biochemical detection system based on a centrifugal microfluidic chip was designed to simplify the rapid detection of renal function indices. A photosensor was used to design an optical signal acquisition structure that can detect products or substrates after enzymatic reactions of uric acid, creatinine, and urea. The weak optical signals collected from this structure were processed using a pre-designed amplifying circuit and a software algorithm to calculate absorbance. The relationship between absorbance and concentration was established according to the Beer-Lambert law. The results indicated good stability and accuracy of the system, which is 21.3 cm × 16.5 cm × 19 cm in size as compared to other detection systems due to the adoption of a centrifugal microfluidic chip. It was portable and easy to operate, in addition to its ability to rapidly detect renal function indices. This system exhibits great potential for the detection of highly integrated point-of-care testing in the future.
Keyphrases
- uric acid
- label free
- circulating tumor cells
- high throughput
- chronic kidney disease
- single cell
- loop mediated isothermal amplification
- metabolic syndrome
- real time pcr
- machine learning
- end stage renal disease
- deep learning
- high resolution
- electronic health record
- current status
- climate change
- nitric oxide
- neural network
- risk assessment
- quantum dots
- sensitive detection