Listeria monocytogenes upregulates mitochondrial calcium signalling to inhibit LC3-associated phagocytosis as a survival strategy.
Tianliang LiLigang KongXinghui LiSijin WuKuldeep S AttriYan LiWeipeng GongBao ZhaoLupeng LiLaura E HerringJohn M AsaraLei XuXiaobo LuoYu Leo LeiQin MaStephanie SeveauJohn S GunnXiaolin ChengPankaj K SinghDouglas R GreenHai-Bo WangHaitao WenPublished in: Nature microbiology (2021)
Mitochondria are believed to have originated ~2.5 billion years ago. As well as energy generation in cells, mitochondria have a role in defence against bacterial pathogens. Despite profound changes in mitochondrial morphology and functions following bacterial challenge, whether intracellular bacteria can hijack mitochondria to promote their survival remains elusive. We report that Listeria monocytogenes-an intracellular bacterial pathogen-suppresses LC3-associated phagocytosis (LAP) by modulation of mitochondrial Ca2+ (mtCa2+) signalling in order to survive inside cells. Invasion of macrophages by L. monocytogenes induced mtCa2+ uptake through the mtCa2+ uniporter (MCU), which in turn increased acetyl-coenzyme A (acetyl-CoA) production by pyruvate dehydrogenase. Acetylation of the LAP effector Rubicon with acetyl-CoA decreased LAP formation. Genetic ablation of MCU attenuated intracellular bacterial growth due to increased LAP formation. Our data show that modulation of mtCa2+ signalling can increase bacterial survival inside cells, and highlight the importance of mitochondrial metabolism in host-microbial interactions.
Keyphrases
- induced apoptosis
- listeria monocytogenes
- oxidative stress
- cell cycle arrest
- reactive oxygen species
- cell death
- endoplasmic reticulum stress
- signaling pathway
- free survival
- gene expression
- high resolution
- big data
- microbial community
- diabetic rats
- endoplasmic reticulum
- dendritic cells
- immune response
- atrial fibrillation
- cell proliferation
- multidrug resistant
- artificial intelligence
- electronic health record
- candida albicans
- intellectual disability
- protein kinase
- endothelial cells