Increased sensitivity of a new commercial reverse transcriptase-quantitative PCR for the detection of Pneumocystis jirovecii in respiratory specimens.

Sarah DellièreSamia HamaneNesrine AissaouiMaud Gits-Muselli Stéphane Bretagne Alexandra Serris

Published in: Medical mycology (2021)

The fungus Pneumocystis needs to be detected early in respiratory samples to prevent pneumonia in immunocompromised hosts. We evaluated a new commercial RTqPCR on 213 respiratory samples to detect Pneumocystis and found it more sensitive and faster than our routine sensitive in-house qPCR assay.

Keyphrases

respiratory tract
high throughput
high resolution
clinical practice
mass spectrometry
label free
loop mediated isothermal amplification
fine needle aspiration
ultrasound guided
mechanical ventilation