Luminescent Device for the Detection of Oxidative Stress Biomarkers in Artificial Urine.
Gopal AmmanathUmit Hakan YildizAlagappan PalaniappanBo LiedbergPublished in: ACS applied materials & interfaces (2018)
A luminescent paper-based device for the visual detection of oxidative stress biomarkers is reported. The device consists of a polyvinylidene fluoride membrane impregnated with poly(3-alkoxy-4-methylthiophene) (PT) for colorimetric detection. 8-hydroxy-2'-deoxyguanosine (8-OHdG), a biomarker associated with oxidative stress, is used as a model system for validating the proposed methodology. The detection strategy is based on monitoring the changes in optical properties of PT associated with its conformational changes upon interaction with an aptamer in the presence and in the absence of 8-OHdG. Fluorometric and colorimetric monitoring revealed linear responses for 8-OHdG concentrations between 50 pM and 500 nM (∼14 pg/mL to 140 ng/mL), with limits of detection of ∼300 pM and ∼350 pM, respectively for ( n = 3). Colorimetric responses in artificial urine ascertained rapid, sensitive, and selective detection of 8-OHdG at clinically relevant (pM to nM) concentration levels. Furthermore, the proposed methodology enables point-of-care diagnostics for oxidative stress without requiring sophisticated instrumentation.
Keyphrases
- oxidative stress
- loop mediated isothermal amplification
- sensitive detection
- label free
- gold nanoparticles
- particulate matter
- real time pcr
- air pollution
- dna damage
- heavy metals
- hydrogen peroxide
- quantum dots
- ischemia reperfusion injury
- fluorescent probe
- living cells
- photodynamic therapy
- molecular dynamics
- high resolution
- molecular dynamics simulations
- single cell
- heat shock