Distinguishing naive- from memory-derived human B cells during acute responses.
Maria AuladellThi H O NguyenBeatriz GarcillánFabienne MackayKatherine KedzierskaAnnette FoxPublished in: Clinical & translational immunology (2019)
Stimulation with R848, IL-21 and sCD40L in the presence of monocytes induces robust differentiation and ASC generation from both naive and memory B-cells. However, naive and memory B cells retain key phenotypic differences after activation that may facilitate ex vivo discrimination and better characterisation of acute responses to variant antigens.