EDTA-K2 Improves the Detection Sensitivity of SARS-CoV-2 IgM and IgG Antibodies by Chelating Colloidal Gold in the Immunochromatographic Assay.

Xiumei HuChangmeng WuBo SituPeifu TianTaixue AnQiang LiWeilun PanRuyi ZhangBiao YangDehua SunYuhai HuQian WangLei Zheng

Published in: International journal of nanomedicine (2021)

GICA is widely used to detect antibodies for the advantages of convenient, fast, low cost, suitable for screening large sample and require minimal equipment. In this study, we found that EDTA-K2 amplified the positive antibody signal by chelating colloidal gold and improved the detection sensitivity of SARS-CoV-2 IgM and IgG antibodies when using the GICA. Therefore, we suggested that EDTA-K2 anticoagulated plasma was more suitable for the detection of SARS-CoV-2 antibodies.

Keyphrases

sars cov
low cost
respiratory syndrome coronavirus
loop mediated isothermal amplification
label free
silver nanoparticles