Salivary IgA and IgG Antibody Responses against Periodontitis-Associated Bacteria in Crohn's Disease.

Elevated serum immunoglobulin (Ig) antibody levels are observed in Crohn's disease patients. The aim of this study was to evaluate the salivary IgA and IgG antibody levels against Porphyromonas gingivalis , Tannerella forsythia , Aggregatibacter actinomycetemcomitans , and Prevotella intermedia in Crohn's disease patients. Eighty-eight participants (47 Crohn's disease patients and 41 systemically healthy age- and gender-matched controls) were included in the study. Oral and medical health statuses were recorded and salivary samples were collected. Salivary P. gingivalis , T. forsythia , A. actinomycetemcomitans , and P. intermedia carriage were analyzed with DNA sequencing technique, salivary levels of IgG1, IgG2, IgG3, IgG4, and IgM were measured with the Luminex ® xMAP™ technique, and salivary IgA and IgG antibody levels against P. gingivalis , T. forsythia , A. actinomycetemcomitans , and P. intermedia were detected by ELISA. As result, higher salivary IgG2 ( p = 0.011) and IgG3 ( p = 0.006), P. gingivalis IgA ( p < 0.001), A. actinomycetemcomitans IgG ( p = 0.001), and P. intermedia IgG ( p < 0.001) antibody levels were detected in the Crohn's disease group compared to the controls. Salivary P. gingivalis carriage was lower in the Crohn's disease group in comparison to the controls ( p = 0.024). In conclusion, salivary IgA antibody responses against P. gingivalis and IgG antibody responses against P. intermedia have independent associations with Crohn's disease.