Activated SOX9+ renal epithelial cells promote kidney repair through secreting factors.
Hao NieZixian ZhaoDewei ZhouDandan LiYujia WangYu MaXutao LiuWei ZuoPublished in: Cell proliferation (2023)
A broad spectrum of lethal kidney diseases involves the irreversible destruction of the tubular structures, leading to renal function loss. Following injury, a spectrum of tissue-resident epithelial stem/progenitor cells are known to be activated and then differentiate into mature renal cells to replace the damaged renal epithelium. Here, however, we reported an alternative way that tissue-resident cells could be activated to secrete multiple factors to promote organ repair. At single-cell resolution, we showed that the resident SOX9+ renal epithelial cells (RECs) could expand in the acutely injured kidney of both mouse and human. Compared to other cells, the SOX9+ RECs overexpressed much more secretion related genes, whose functions were linked to kidney repair pathways. We also obtained long-term, feeder-free cultured SOX9+ RECs from human urine and analysed their secretory profile at both transcriptional and proteomic levels. Engraftment of cultured human SOX9+ RECs or injection of its conditional medium facilitated the regeneration of renal tubular and glomerular epithelium, probably through stimulating endogenous REC self-activation and mediating crosstalk with other renal cells. We also identified S100A9 as one of the key factors in the SOX9+ REC secretome. Altogether, the abilities to extensively propagate SOX9+ RECs in culture whilst concomitantly maintaining their intrinsic secretory capacity suggest their future application in cell-free therapies and regeneration medicine.
Keyphrases
- stem cells
- induced apoptosis
- transcription factor
- endothelial cells
- cell cycle arrest
- single cell
- cell free
- endoplasmic reticulum stress
- oxidative stress
- high glucose
- gene expression
- induced pluripotent stem cells
- multidrug resistant
- single molecule
- cell proliferation
- diabetic nephropathy
- circulating tumor cells
- heat shock protein