Adherence of Trichomonas vaginalis to SiHa Cells is Inhibited by Diphenyleneiodonium.
Yeeun KimYoung Ha LeeIn-Wook ChoiBu Yeon HeoJu-Gyeong KangJae-Min YukGuang-Ho ChaEun-Kyeong JoJaeyul KwonPublished in: Microorganisms (2020)
Microbial adhesion is critical for parasitic infection and colonization of host cells. To study the host-parasite interaction in vitro, we established a flow cytometry-based assay to measure the adherence of Trichomonas vaginalis to epithelial cell line SiHa. SiHa cells and T. vaginalis were detected as clearly separated, quantifiable populations by flow cytometry. We found that T. vaginalis attached to SiHa cells as early as 30 min after infection and the binding remained stable up to several hours, allowing for analysis of drug treatment efficacy. Importantly, NADPH oxidase inhibitor DPI treatment induced the detachment of T. vaginalis from SiHa cells in a dose-dependent manner without affecting host cell viability. Thus, this study may provide an understanding for the potential development of therapies against T. vaginalis and other parasite infections.
Keyphrases
- induced apoptosis
- cell cycle arrest
- flow cytometry
- oxidative stress
- emergency department
- cell death
- adipose tissue
- escherichia coli
- risk assessment
- microbial community
- climate change
- pi k akt
- skeletal muscle
- candida albicans
- weight loss
- high throughput
- insulin resistance
- single cell
- binding protein
- toxoplasma gondii
- cell migration
- replacement therapy