Highly Sensitive EGFRvIII Detection in Circulating Extracellular Vesicle RNA of Glioma Patients.
Syeda Maheen BatoolKoushik MuralidharanTiffaney HsiaSarah FaloticoAustin S GamblinYulia B RosenfeldSirena K KhannaLeonora BalajBob S CarterPublished in: Clinical cancer research : an official journal of the American Association for Cancer Research (2022)
This study demonstrates the feasibility of detecting EGFRvIII mutation in plasma using a highly sensitive and specific ddPCR assay. We also show a higher than previously reported EGFRvIII prevalence in glioma tumor tissue. Several features of the assay are favorable for clinical implementation for detection and monitoring of EGFRvIII-positive tumors.
Keyphrases
- end stage renal disease
- label free
- high throughput
- ejection fraction
- chronic kidney disease
- newly diagnosed
- loop mediated isothermal amplification
- healthcare
- primary care
- risk factors
- real time pcr
- prognostic factors
- peritoneal dialysis
- fluorescent probe
- living cells
- patient reported outcomes
- single cell
- sensitive detection
- quantum dots
- single molecule
- liquid chromatography