Characterization of genes involved in micronutrients and toxic metals detoxification in Brassica napus by genome-wide cDNA library screening.
Jia-Shi PengXue-Jie ZhangJia-Ni XiongYing ZhouWei-Li WangSi-Ying ChenDa-Wei ZhangTian-Yu GuPublished in: Metallomics : integrated biometal science (2023)
Stresses caused by deficiency/excess of mineral nutrients or of pollution of toxic metals have already become a primary factor in limiting crop production worldwide. Genes involved in minerals and toxic metals accumulation/tolerance could be potential candidates for improving crop plants with enhanced nutritional efficiency and environmental adaptability. In this study, we first generated a high-quality yeast expression cDNA library of Brassica napus (Westar), and 46 genes mediating excess micronutrients and toxic metals detoxification were screened using the yeast genetic complementation system, including 11, 5, 6, 14, 6, 5 genes involved in cadmium (Cd), zinc (Zn), iron (Fe), manganese (Mn), boron (B), copper (Cu) tolerance respectively. Characterization of genes mediating excess ions stress resistance in this study is beneficial for us to further understand ions homeostasis in B. napus.
Keyphrases
- human health
- genome wide
- heavy metals
- health risk assessment
- risk assessment
- climate change
- dna methylation
- genome wide identification
- genome wide analysis
- quantum dots
- aqueous solution
- oxide nanoparticles
- copy number
- gene expression
- transcription factor
- drinking water
- air pollution
- smoking cessation
- replacement therapy