Detection of Group A Streptococcus in Pharyngeal Swab Specimens by Use of the AmpliVue GAS Isothermal Helicase-Dependent Amplification Assay.

Matthew L FaronNathan A LedeboerPaul GranatoJudy A DalyKristina PiercePreeti PancholiTimothy S UphoffBlake W Buchan

Published in: Journal of clinical microbiology (2015)

We evaluated the clinical performance (sensitivity and specificity) of the AmpliVue group A Streptococcus (GAS) isothermal helicase-dependent amplification assay using 1,192 pharyngeal swab specimens. AmpliVue GAS assay results were compared to the results of routine throat cultures on selective streptococcal blood agar plates. The sensitivity and specificity of the AmpliVue GAS assay were 98.3% (95% confidence interval [CI], 95 to 100%) and 93.2% (95% CI, 91 to 95%), respectively.

Keyphrases

high throughput
nucleic acid
room temperature
biofilm formation
label free
carbon dioxide
clinical practice
escherichia coli
pseudomonas aeruginosa
cystic fibrosis
single cell