Thermophilic Exopolysaccharide Films: A Potential Device for Local Antibiotic Delivery.
Joseph M LaubachRajesh Kumar SaniPublished in: Pharmaceutics (2023)
Natural polysaccharides being investigated for use in the field of drug delivery commonly require the addition of sugars or pretreated biomass for fabrication. Geobacillus sp. strain WSUCF1 is a thermophile capable of secreting natural polymers, termed exopolysaccharides (EPSs), cultivated from cost-effective, non-treated lignocellulosic biomass carbon substrates. This preliminary investigation explores the capabilities of a 5% wt/wt amikacin-loaded film constructed from the crude EPS extracted from the strain WSUCF1. Film samples were seen to be non-cytotoxic to human keratinocytes and human skin-tissue fibroblasts, maintaining cell viability, on average, above 85% for keratinocytes over 72-h during a cell viability assay. The drug release profile of a whole film sample revealed a steady release of the antibiotic up to 12 h. The amikacin eluted by the EPS film was seen to be active against Staphylococcus aureus , maintaining above a 91% growth inhibition over a period of 48 h. Overall, this study demonstrates that a 5% amikacin-EPS film, grown from lignocellulosic biomass, can be a viable option for preventing or combating infections in clinical treatment.
Keyphrases
- drug delivery
- anaerobic digestion
- room temperature
- drug release
- reduced graphene oxide
- wastewater treatment
- staphylococcus aureus
- endothelial cells
- cancer therapy
- wound healing
- gold nanoparticles
- single cell
- risk assessment
- ionic liquid
- extracellular matrix
- high resolution
- newly diagnosed
- mass spectrometry
- high speed
- methicillin resistant staphylococcus aureus