Systemic and Renal Dynamics of Free Sulfhydryl Groups during Living Donor Kidney Transplantation.
Nora A SpraakmanAnnemieke M CoesterArno R BourgonjeVincent B NieuwenhuijsJan-Stephan F SandersHenri G D LeuveninkHarry Van GoorGertrude J Nieuwenhuijs-MoekePublished in: International journal of molecular sciences (2022)
During ischemia-reperfusion injury (IRI), reactive oxygen species are produced that can be scavenged by free sulfhydryl groups (R-SH, free thiols). In this study, we hypothesized that R-SH levels decrease as a consequence of renal IRI and that R-SH levels reflect post-transplant graft function. Systemic venous, arterial, renal venous, and urinary samples were collected in donors and recipients before, during, and after transplantation. R-SH was measured colorimetrically. Systemic arterial R-SH levels in recipients increased significantly up to 30 sec after reperfusion ( p < 0.001). In contrast, renal venous R-SH levels significantly decreased at 5 and 10 min compared to 30 sec after reperfusion (both p < 0.001). This resulted in a significant decrease in delta R-SH (defined as the difference between renal venous and systemic arterial R-SH levels) till 30 sec after reperfusion ( p < 0.001), indicating a net decrease in R-SH levels across the transplanted kidney. Overall, these results suggest trans-renal oxidative stress as a consequence of IRI during kidney transplantation, reflected by systemic and renal changes in R-SH levels in transplant recipients.
Keyphrases
- kidney transplantation
- oxidative stress
- ischemia reperfusion injury
- acute myocardial infarction
- reactive oxygen species
- magnetic resonance imaging
- stem cells
- magnetic resonance
- dna damage
- cerebral ischemia
- computed tomography
- coronary artery disease
- signaling pathway
- left ventricular
- drug induced
- acute coronary syndrome
- cell therapy