Iridium-Triggered Allylcarbamate Uncaging in Living Cells.
Neelu SinghAjay GuptaPuja PrasadPritam MahawarShalini GuptaPijus K SasmalPublished in: Inorganic chemistry (2021)
Designing a metal catalyst that addresses the major issues of solubility, stability, toxicity, cell uptake, and reactivity within complex biological milieu for bioorthogonal controlled transformation reactions is a highly formidable challenge. Herein, we report an organoiridium complex that is nontoxic and capable of the uncaging of allyloxycarbonyl-protected amines under biologically relevant conditions and within living cells. The potential applications of this uncaging chemistry have been demonstrated by the generation of diagnostic and therapeutic agents upon the activation of profluorophore and prodrug in a controlled fashion within HeLa cells, providing a valuable tool for numerous potential biological and therapeutic applications.
Keyphrases
- living cells
- fluorescent probe
- single molecule
- cell cycle arrest
- induced apoptosis
- oxidative stress
- single cell
- cell therapy
- human health
- cancer therapy
- highly efficient
- cell death
- risk assessment
- stem cells
- endoplasmic reticulum stress
- gold nanoparticles
- water soluble
- drug delivery
- drug release
- reduced graphene oxide