Rapid Unperturbed-Tissue Analysis for Intraoperative Cancer Diagnosis Using an Enzyme-Activated NIR-II Nanoprobe.
Yang ZhanSisi LingHaoying HuangYejun ZhangGuangcun ChenShungen HuangChunyan LiWanliang GuoQiangbin WangPublished in: Angewandte Chemie (International ed. in English) (2020)
Accurate intraoperative tissue identification is critical to tumor surgery. However, conventional methods are labor- and time-intensive, which greatly delay the intraoperative decision-making. Herein, a matrix metalloproteinase (MMP)14-activated NIR-II nanoprobe (A&MMP@Ag2 S-AF7P) is presented for rapid unperturbed-tissue analysis for ex vivo and in vivo neuroblastoma diagnosis. A&MMP@Ag2 S-AF7P displays negligible fluorescence in normal tissues but is activated quickly by inhibiting the fluorescence resonance energy transfer (FRET) between Ag2 S QDs and A1094 mediated by MMP14 overexpressed in neuroblastoma; meanwhile, the exposure of the membrane penetrating peptide R9 (TAT-peptide) results in efficient internalization of nanoprobes in the cancer cells, providing superior tumor-to-normal (T/N) tissue ratio. Instant illumination of the lesion and well-defined tumor margins make the nanoprobes a suitable rapid diagnostic reagent for cancer surgical or tissue biopsy procedures.
Keyphrases
- energy transfer
- quantum dots
- fluorescence imaging
- decision making
- minimally invasive
- single molecule
- atrial fibrillation
- cell migration
- papillary thyroid
- photodynamic therapy
- fluorescent probe
- gene expression
- squamous cell carcinoma
- living cells
- squamous cell
- high resolution
- signaling pathway
- lymph node metastasis
- coronary artery bypass