MicroRNA-6869-5p acts as a tumor suppressor via targeting TLR4/NF-κB signaling pathway in colorectal cancer.
Shushan YanGuoyan LiuChengwen JinZengfang WangQuanhong DuanJiang XuDonghua XuPublished in: Journal of cellular physiology (2018)
Many studies have implicated that microRNAs (miRNAs), as non-coding RNAs, play important roles in the development and progression of colorectal cancer (CRC). However, little is known about the role of a newly identified miRNA, miR-6869-5p, in CRC. We aim to investigate the modifying effects and underlying mechanisms of miR-6869-5 in colorectal carcinogenesis and progression. Significantly reduced levels of miR-6869-5p were observed in both serum exosomes tumor tissue samples from patients with CRC. The prediction of targets of miR-6869-5p in databases of targetscan, microRNA. ORG and miRDBA revealed that toll-like receptor 4 (TLR4) is a potential target for this miRNA. MiR-6869-5p could inhibit cell proliferation and the production of inflammatory cytokines (TNF-α and IL-6) in CRC cells via directly targeting TLR4. The protective effect of miR-6869-5p from colorectal carcinogenesis was dependent on TLR4/NF-κB signaling pathway. In addition, the 3-year survival was poor among CRC patients with decreased levels of miR-6869-5p in serum exosomes. Thus, miR-6869-5p may serve as a tumor suppressor in CRC, and serum exosomal miR-6869-5p is a promising circulating biomarker for the prediction of CRC prognosis.
Keyphrases
- toll like receptor
- signaling pathway
- nuclear factor
- inflammatory response
- pi k akt
- cell proliferation
- induced apoptosis
- immune response
- lps induced
- cell cycle arrest
- mesenchymal stem cells
- stem cells
- rheumatoid arthritis
- epithelial mesenchymal transition
- long non coding rna
- cancer therapy
- risk assessment
- climate change
- cell death
- drug delivery
- human health