Immunophenotypic characterization of leukemic stem cells in acute myeloid leukemia using single tube 10-colour panel by multiparametric flow cytometry: Deciphering the spectrum, complexity and immunophenotypic heterogeneity.
Nupur DasDevasis PandaSmeeta GajendraRitu GuptaDeepshi ThakralGurvinder KaurAafreen KhanVivek Kumar SinghArushi VempralaSameer BakhshiRachna SethRanjit Kumar SahooAtul SharmaSandeep RaiVijay K PrajapatiSaroj SinghPublished in: International journal of laboratory hematology (2024)
A single tube-10-colour MFC panel is proposed as an easy and reproducible tool to identify and discriminate LSCs from HSCs. LSCs display both inter- and intra-sample heterogeneity in terms of antigen expressions, which opens the facets for single cell molecular analysis to elucidate the role of subpopulations of LSCs in AML progression.