A new PacBio genome sequence of an Australian Pyrenophora tritici-repentis race 1 isolate.
Paula M MoolhuijzenPao Theen SeeCaroline S MoffatPublished in: BMC research notes (2019)
Over 1.3 million reads were sequenced by PacBio Sequel small-molecule real-time sequencing (SRMT) cell to yield 11.4 Gb for the genome assembly of V1 (285X coverage), with median and maximum read lengths of 8959 bp and 72,292 bp respectively. The V1 genome was assembled into 33 contiguous sequences with a of total length 40.4 Mb and GC content of 50.44%. A total of 14,050 protein coding genes were predicted and annotated for V1. Of these 11,519 genes were orthologous to both Pt-1C-BFP and M4. Whole genome alignment of the Australian long-read assemblies (V1 to M4) confirmed previously identified large-scale genome rearrangements between M4 and Pt-1C-BFP and presented small scale variations, which included a sequence break within a race-specific region for ToxA, a well-known necrotrophic effector gene.
Keyphrases
- genome wide
- small molecule
- dna methylation
- genome wide identification
- single cell
- copy number
- protein protein
- genome wide analysis
- amino acid
- cell therapy
- healthcare
- transcription factor
- regulatory t cells
- bioinformatics analysis
- stem cells
- bone marrow
- mesenchymal stem cells
- high resolution
- liquid chromatography
- simultaneous determination