Intrinsically Disordered Protein Condensate-Modified Surface for Mitigation of Biofouling and Foreign Body Response.
Rong ChangJia-Lin ChenGuan-Yi ZhangYue LiHua-Zhen DuanShi-Zhong LuoYong Xiang ChenPublished in: Journal of the American Chemical Society (2022)
Mitigation of biofouling and the host's foreign body response (FBR) is a critical challenge with biomedical implants. The surface coating with various anti-fouling materials provides a solution to overcome it, but limited options in clinic and their potential immunogenicity drive the development of more alternative coating materials. Herein, inspired by liquid-liquid phase separation of intrinsically disordered proteins (IDPs) to form separated condensates in physiological conditions, we develop a new type of low-fouling biomaterial based on flexible IDP of FUS protein containing rich hydrophilic residues. A chemical structure-defined FUS IDP sequence tagged with a tetra-cysteine motif (IDP FUS ) was engineered and applied for covalent immobilization on various surfaces to form a uniform layer of protein tangles, which boosted strong hydration on surfaces, as revealed by molecular dynamics simulation. The IDP FUS -coated surfaces displayed excellent performance in resisting adsorption of various proteins and adhesion of different cells, platelets, and bacteria. Moreover, the IDP FUS -coated implants largely mitigated the host's FBR compared with bare implants and particularly outperformed PEG-coated implants in reducing collagen encapsulation. Thus, this novel low-fouling and anti-FBR strategy provides a potential surface coating material for biomedical implants, which will also shed light on exploring similar applications of other IDP proteins.
Keyphrases
- soft tissue
- molecular dynamics simulations
- biofilm formation
- amino acid
- climate change
- protein protein
- induced apoptosis
- staphylococcus aureus
- primary care
- drug delivery
- escherichia coli
- liquid chromatography
- cystic fibrosis
- pseudomonas aeruginosa
- mass spectrometry
- oxidative stress
- signaling pathway
- risk assessment
- endoplasmic reticulum stress
- fluorescent probe
- aqueous solution
- living cells
- tissue engineering
- candida albicans
- solid phase extraction