Modular Engineering of DNAzyme-Based Sensors for Spatioselective Imaging of Metal Ions in Mitochondria.
Deyu YiHengzhi ZhaoJian ZhaoLele LiPublished in: Journal of the American Chemical Society (2022)
DNAzyme-based sensors remain at the forefront of metal-ion imaging efforts, but most lack the subcellular precision necessary to their applications in specific organelles. Here, we seek to overcome this limitation by presenting a DNAzyme-based biosensor technology for spatiotemporally controlled imaging of metal ions in mitochondria. A DNA nanodevice was constructed by integrating an optically activatable DNAzyme sensor and an upconversion nanoparticle with an organelle-targeting signal. We exemplify that this approach allows for mitochondria-specific imaging of Zn 2+ in living cells in a near-infrared light-controlled manner. Based on this, the system is used for the monitoring of mitochondrial Zn 2+ during drug treatment in a cellular model of ischemia insult. Furthermore, the DNA nanodevice is employed to assess dynamic Zn 2+ change and pharmacological interventions in an injury cell model of Zn 2+ toxicity. This method paves the way for engineering of DNAzyme sensors to investigate the pathophysiological roles of metal ions at the subcellular level.
Keyphrases
- living cells
- fluorescent probe
- high resolution
- single molecule
- label free
- heavy metals
- quantum dots
- cell death
- oxidative stress
- emergency department
- fluorescence imaging
- stem cells
- photodynamic therapy
- physical activity
- reactive oxygen species
- low cost
- single cell
- risk assessment
- wastewater treatment
- cell free
- mesenchymal stem cells
- drug delivery
- replacement therapy