Phenylboronic Acid Templated Gold Nanoclusters for Mucin Detection Using a Smartphone-Based Device and Targeted Cancer Cell Theranostics.
Deepanjalee DuttaSunil Kumar SailapuArun ChattopadhyaySiddhartha Sankar GhoshPublished in: ACS applied materials & interfaces (2018)
A phenylboronic acid templated gold nanocluster probe was developed to detect biomarker mucin by a noninvasive fluorescence-based method using a point-of-care smartphone-based fluorescence detection device. The gold nanocluster probe is able to detect mucin specifically. The same probe was applied for in vitro targeted bioimaging of HeLa and Hep G2 cancer cells, and it demonstrated specific therapeutic effects toward cancer cells as well as multicellular tumor spheroids imparting theranostic properties. The module is found to be more effective toward HeLa cells, and a pathway of cell death was established using flow-cytometry-based assays.
Keyphrases
- cell cycle arrest
- cell death
- living cells
- quantum dots
- flow cytometry
- fluorescent probe
- energy transfer
- single molecule
- label free
- loop mediated isothermal amplification
- pi k akt
- sensitive detection
- cancer therapy
- induced apoptosis
- real time pcr
- silver nanoparticles
- signaling pathway
- photodynamic therapy
- high throughput
- endoplasmic reticulum stress
- fluorescence imaging