Functionalized Ionic Liquids-Modified Metal-Organic Framework Material Boosted the Enzymatic Performance of Lipase.
Liran JiWei ZhangYifei ZhangYuan-Fa LiuYi HuPublished in: Molecules (Basel, Switzerland) (2024)
The development of immobilized enzymes with high activity and stability is critical. Metal-organic frameworks (MOFs) have attracted much academic and industrial interest in the field of enzyme immobilization due to their unique properties. In this study, the amino-functionalized ionic liquid (NIL)-modified metal-organic framework (UiO-66-NH 2 ) was prepared to immobilize Candida rugosa lipase (CRL), using dialdehyde starch (DAS) as the cross-linker. The results of the Fourier transform infrared (FT-IR) spectra, X-ray powder diffraction (XRD), and scanning electronic microscopy (SEM) confirmed that the NIL was successfully grafted to UiO-66-NH 2 . The CRL immobilized on NIL-modified UiO-66-NH 2 (UiO-66-NH 2 -NIL-DAS@CRL) exhibited satisfactory activity recovery (79.33%), stability, reusability, and excellent organic solvent tolerance. The research results indicated that ionic liquid-modified UiO-66-NH 2 had practical potential for application in enzyme immobilization.
Keyphrases
- metal organic framework
- ionic liquid
- room temperature
- high resolution
- disease activity
- quantum dots
- perovskite solar cells
- heavy metals
- single molecule
- rheumatoid arthritis
- hydrogen peroxide
- nitric oxide
- computed tomography
- pseudomonas aeruginosa
- molecularly imprinted
- magnetic resonance
- wastewater treatment
- candida albicans
- high speed
- dual energy