First-time application of droplet digital PCR for methylation testing of the 11p15.5 imprinting regions.

Elia SchlaichWouter H G HubensEggermann Thomas

Published in: Molecular genetics & genomic medicine (2023)

We show for the first time that MS-ddPCR is a reliable and easy applicable method for determination of MS-associated changes in imprinting disorders. It is therefore an additional tool for multimethod diagnostics of imprinting disorders suitable to improve the diagnostic yield.

Keyphrases

mass spectrometry
multiple sclerosis
ms ms
dna methylation
high throughput
genome wide
solid phase extraction
molecularly imprinted
gene expression
liquid chromatography
simultaneous determination