Engineered flavoproteins as bioorthogonal photo-triggers for the activation of metal-based anticancer prodrugs.

Laura F Mazzei Juan Gurruchaga-Pereda Alvaro Jose Martinez-Valencia Javier Calvo Martínez Luca Salassa Aitziber L Cortajarena

Published in: Chemical communications (Cambridge, England) (2023)

A multifunctional hybrid constructed for controlling the delivery and activation of Pt anticancer agents in vitro is described herein. We employed consensus tetratricopeptide repeat protein (CTPR) for the covalent co-anchoring of riboflavin (photocatalyst) and a Pt(IV) prodrug complex. The Pt-loaded flavoprotein induced a 40% reduction in PANC-1 cell viability as a result of the photocatalytic formation of cisplatin.

Keyphrases

cancer therapy
drug delivery
visible light
highly efficient
high glucose
diabetic rats
wastewater treatment
drug induced
small molecule
binding protein
clinical practice
oxidative stress
amino acid
endothelial cells
reduced graphene oxide
gold nanoparticles