Identification of PKS-NRPS Hybrid Metabolites in Marine-Derived Penicillium oxalicum .
Hongcheng LiWei ZhangXuan ZhangShen TangPing MenMengyi XiongZhi-Min LiYongyu ZhangXuenian HuangXuefeng LuPublished in: Marine drugs (2022)
Filamentous fungi are abundant resources of bioactive natural products. Here, 151 marine-derived fungi were collected from the north Yellow Sea and identified by an internal transcribed spacer (ITS) sequence. The crude extracts of all strains were evaluated for their antimicrobial activities and analyzed by HPLC fingerprint. Based on these, strain Penicillium oxalicum MEFC104 was selected for further investigation. Two new polyketide-amino acid hybrid compounds with feature structures of tetramic acid, oxopyrrolidine A and B, were isolated. Their planner structures were assigned by HRESIMS and 1D/2D NMR experiments. The absolute configurations were determined by modified Mosher's method, J -based configuration analysis, and ECD calculations. Furthermore, the biosynthetic pathway was identified by bioinformatic analysis and gene-deletion experiments. This study established a link between oxopyrrolidines and the corresponding biosynthesis genes in P . oxalicum .
Keyphrases
- high resolution
- ms ms
- amino acid
- genome wide
- escherichia coli
- machine learning
- staphylococcus aureus
- magnetic resonance
- mass spectrometry
- gene expression
- bioinformatics analysis
- copy number
- high performance liquid chromatography
- molecular dynamics
- dna methylation
- transcription factor
- tertiary care
- genome wide identification
- tandem mass spectrometry
- solid phase extraction
- monte carlo