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Methanobrevibacter massiliense and Pyramidobacter piscolens Co-Culture Illustrates Transkingdom Symbiosis.

Virginie PilliolMamadou BeyeLaureline TerlierJulien BalmelleIdir KacelRomain LanGérard AboudharamGhiles GrineElodie Terrer
Published in: Microorganisms (2024)
Among oral microbiota methanogens, Methanobrevibacter massiliense ( M. massiliense ) has remained less studied than the well-characterised and cultivated methanogens Methanobrevibacter oralis and Methanobrevibacter smithii . M. massiliense has been associated with different oral pathologies and was co-isolated with the Synergistetes bacterium Pyramidobacter piscolens ( P. piscolens ) in one case of severe periodontitis. Here, reporting on two additional necrotic pulp cases yielded the opportunity to characterise two co-cultivated M. massiliense isolates, both with P. piscolens , as non-motile, 1-2-µm-long and 0.6-0.8-µm-wide Gram-positive coccobacilli which were autofluorescent at 420 nm. The two whole genome sequences featured a 31.3% GC content, gapless 1,834,388-base-pair chromosome exhibiting an 85.9% coding ratio, encoding a formate dehydrogenase promoting M. massiliense growth without hydrogen in GG medium. These data pave the way to understanding a symbiotic, transkingdom association with P. piscolens and its role in oral pathologies.
Keyphrases
  • emergency department
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  • mass spectrometry
  • machine learning
  • gene expression
  • big data
  • genetic diversity
  • dna methylation
  • multidrug resistant
  • solid state
  • visible light