Endoplasmic reticulum stress-induced apoptosis in the penumbra aggravates secondary damage in rats with traumatic brain injury.
Guo-Zhu SunFen-Fei GaoZong-Mao ZhaoHai SunWei XuLi-Wei WuYong-Chang HePublished in: Neural regeneration research (2016)
Neuronal apoptosis is mediated by intrinsic and extrinsic signaling pathways such as the membrane-mediated, mitochondrial, and endoplasmic reticulum stress pathways. Few studies have examined the endoplasmic reticulum-mediated apoptosis pathway in the penumbra after traumatic brain injury, and it remains unclear whether endoplasmic reticulum stress can activate the caspase-12-dependent apoptotic pathway in the traumatic penumbra. Here, we established rat models of fluid percussion-induced traumatic brain injury and found that protein expression of caspase-12, caspase-3 and the endoplasmic reticulum stress marker 78 kDa glucose-regulated protein increased in the traumatic penumbra 6 hours after injury and peaked at 24 hours. Furthermore, numbers of terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling-positive cells in the traumatic penumbra also reached peak levels 24 hours after injury. These findings suggest that caspase-12-mediated endoplasmic reticulum-related apoptosis is activated in the traumatic penumbra, and may play an important role in the pathophysiology of secondary brain injury.
Keyphrases
- induced apoptosis
- endoplasmic reticulum stress
- endoplasmic reticulum
- traumatic brain injury
- brain injury
- spinal cord injury
- oxidative stress
- subarachnoid hemorrhage
- cell death
- signaling pathway
- diabetic rats
- cerebral ischemia
- blood pressure
- adipose tissue
- epithelial mesenchymal transition
- endothelial cells
- skeletal muscle
- severe traumatic brain injury