Bioprinted Osteogenic and Vasculogenic Patterns for Engineering 3D Bone Tissue.
Batzaya ByambaaNasim AnnabiKan YueGrissel Trujillo-de SantiagoMario Moisés AlvarezWeitao JiaMehdi Kazemzadeh-NarbatSu Ryon ShinAli TamayolStefano PasseriniPublished in: Advanced healthcare materials (2017)
Fabricating 3D large-scale bone tissue constructs with functional vasculature has been a particular challenge in engineering tissues suitable for repairing large bone defects. To address this challenge, an extrusion-based direct-writing bioprinting strategy is utilized to fabricate microstructured bone-like tissue constructs containing a perfusable vascular lumen. The bioprinted constructs are used as biomimetic in vitro matrices to co-culture human umbilical vein endothelial cells and bone marrow derived human mesenchymal stem cells in a naturally derived hydrogel. To form the perfusable blood vessel inside the bioprinted construct, a central cylinder with 5% gelatin methacryloyl (GelMA) hydrogel at low methacryloyl substitution (GelMALOW ) was printed. We also develop cell-laden cylinder elements made of GelMA hydrogel loaded with silicate nanoplatelets to induce osteogenesis, and synthesized hydrogel formulations with chemically conjugated vascular endothelial growth factor to promote vascular spreading. It was found that the engineered construct is able to support cell survival and proliferation during maturation in vitro. Additionally, the whole construct demonstrates high structural stability during the in vitro culture for 21 days. This method enables the local control of physical and chemical microniches and the establishment of gradients in the bioprinted constructs.
Keyphrases
- endothelial cells
- mesenchymal stem cells
- drug delivery
- vascular endothelial growth factor
- bone regeneration
- bone mineral density
- hyaluronic acid
- tissue engineering
- wound healing
- soft tissue
- bone loss
- bone marrow
- umbilical cord
- cell therapy
- physical activity
- signaling pathway
- gene expression
- mental health
- body composition
- single cell
- high glucose
- stem cells
- ultrasound guided