Field-applicable simultaneous multiplex LAMP assay for screening HBV and HCV co-infection in a single tube.

Published in: BMC infectious diseases (2024)

The mLAMP assay displays significant promise for analyzing coinfected samples by simultaneously detecting the dual targets HBV and HCV within a set temperature of 62 °C, all within a time frame of 1 h. Additionally, when paired with disposable LFD, the mLAMP assay enables rapid visual detection of assay results in a matter of minutes. The result contributes to the mLAMP assay being highly suitable for coinfection screening, particularly in field conditions.

Keyphrases

high throughput
loop mediated isothermal amplification
hepatitis b virus
human immunodeficiency virus
machine learning
quantum dots
hiv infected
sensitive detection