Development and Application of Real-Time PCR-Based Screening for Identification of Omicron SARS-CoV-2 Variant Sublineages.
Anna EsmanDmitry DubodelovKamil KhafizovIvan KotovGerman V RoevAnna GolubevaGasan GasanovMarina KorabelnikovaAskar TurashevEvgeniy CherkashinKonstantin MironovAnna S CherkashinaVasiliy Gennadevich AkimkinPublished in: Genes (2023)
The Omicron strain is currently the main dominant variant of SARS-CoV-2, with a large number of sublineages. In this article, we present our experience in tracing it in Russia using molecular diagnostic methods. For this purpose, different approaches were used; for example, we developed multiprimer panels for RT-PCR and Sanger and NGS sequencing methods. For the centralized collection and analysis of samples, the VGARus database was developed, which currently includes more than 300,000 viral sequences.