One-Step Thermophoretic AND Gate Operation on Extracellular Vesicles Improves Diagnosis of Prostate Cancer.
Jinqi DengShuai ZhaoJunhong LiYangchang ChengChao LiuZheng LiuLele LiFei TianBo DaiJiashu SunPublished in: Angewandte Chemie (International ed. in English) (2022)
Circulating extracellular vesicles (EVs) have emerged as a valuable source of cancer biomarkers. However, the high degree of EV heterogeneity and the complexity of clinical samples pose a challenge in the sensitive identification of tumor-derived EVs. Here we introduce a one-step thermophoretic AND gate operation (Tango) assay that integrates polyethylene glycol (PEG)-enhanced thermophoretic accumulation of EVs and simultaneous AND gate operation on EV membranes by dual-aptamers recognition. By using the Tango assay to detect tumor-derived EVs with co-presence of EpCAM and PSMA directly from serum in a homogeneous, separation-free format, we can discriminate prostate cancer (PCa) patients from benign prostatic hyperplasia (BPH) patients in the diagnostic gray zone with an accuracy of 91 % in 15 min. Our approach streamlines EV enrichment and AND gate operation on EVs in a single assay, providing a rapid, straightforward, and powerful method for precise and non-invasive diagnosis of cancer.
Keyphrases
- prostate cancer
- end stage renal disease
- benign prostatic hyperplasia
- ejection fraction
- newly diagnosed
- chronic kidney disease
- high throughput
- lower urinary tract symptoms
- papillary thyroid
- prognostic factors
- radical prostatectomy
- drug delivery
- computed tomography
- pet ct
- patient reported outcomes
- single cell
- young adults
- quantum dots