Fabrication of an electrochemical biosensor with ZnO nanoflakes interface for methylglyoxal quantification in food samples.
Amudha JayaprakasanAmarnath ThangavelLakshmishri PrabakaranManju Bhargavi GumpuNoel NesakumarK Jayanth BabuSrinivasan VedanthamJohn Bosco Balaguru RayappanPublished in: Food science and biotechnology (2017)
Increased consumption of fried foods such as grilled chicken contains elevated levels of methylglyoxal (MG), which is associated with diabetes mellitus. Hence, in this work, glyoxalase 1(GLO 1) based, zinc oxide (ZnO) flakes interfaced mediator free electrochemical biosensor was developed to detect MG in grilled chicken. ZnO flakes were synthesized by direct precipitation method. X-ray diffractometer and field emission scanning electron microscope were used to study the structural and morphological characteristics of ZnO flakes. The immobilization of GLO 1 on Pt/ZnO flakes modified electrode was confirmed by Fourier transform infrared spectroscopy. Cyclic voltammetric and amperometric studies were carried out using Pt/ZnO flakes/GLO 1 working electrode. The developed biosensor exhibited linear range of 0.6-2.0 µM, sensitivity of 0.281 µA µM-1, LOD of 9 nM with a response time of <4 s and shelf life of 18 days (89%).
Keyphrases
- quantum dots
- reduced graphene oxide
- gold nanoparticles
- room temperature
- sensitive detection
- label free
- visible light
- light emitting
- ionic liquid
- high resolution
- molecularly imprinted
- photodynamic therapy
- metabolic syndrome
- type diabetes
- electron microscopy
- mass spectrometry
- magnetic resonance imaging
- oxide nanoparticles
- nitric oxide
- insulin resistance
- neural network