Rapid Sample Screening Method for Authenticity Controlling of Vanilla Flavours Using Liquid Chromatography with Electrochemical Detection Using Aluminium-Doped Zirconia Nanoparticles-Modified Electrode.
Yassine BenmassaoudKhaled MurtadaRachid SalghiMohammed ZougaghAngel RíosPublished in: Molecules (Basel, Switzerland) (2022)
A rapid and sensitive technique for frauds determination in vanilla flavors was developed. The method comprises separation by liquid chromatography followed by an electrochemical detection using a homemade screen-printed carbon electrode modified with aluminium-doped zirconia nanoparticles (Al-ZrO 2 -NPs/SPCE). The prepared nanomaterials (Al-ZrO 2 -NPs) were characterized by using X-ray diffraction (XRD), transmission electron microscopy (TEM) and energy dispersive X-ray (EDX). This method allows for the determination of six phenolic compounds of vanilla flavors, namely, vanillin, p-hydroxybenzoic acid, p-hydroxybenzaldehyde, vanillyl alcohol, vanillic acid and ethyl vanillin in a linear range between 0.5 and 25 µg g -1 , with relative standard deviation values from 2.89 to 4.76%. Meanwhile, the limits of detection and quantification were in the range of 0.10 to 0.14 µg g -1 and 0.33 to 0.48 µg g -1 , respectively. In addition, the Al-ZrO 2 -NPs/SPCE method displayed a good reproducibility, high sensitivity, and good selectivity towards the determination of the vanilla phenolic compounds, making it suitable for the determination of vanilla phenolic compounds in vanilla real extracts products.
Keyphrases
- solid phase extraction
- liquid chromatography
- molecularly imprinted
- loop mediated isothermal amplification
- electron microscopy
- label free
- tandem mass spectrometry
- mass spectrometry
- high resolution mass spectrometry
- ionic liquid
- simultaneous determination
- gas chromatography mass spectrometry
- quantum dots
- gold nanoparticles
- high resolution
- gas chromatography
- sensitive detection
- magnetic resonance
- high throughput
- highly efficient
- carbon nanotubes
- magnetic resonance imaging
- computed tomography
- oxide nanoparticles
- alcohol consumption
- solid state