A Fluorescent Sensor-Assisted Paper-Based Competitive Lateral Flow Immunoassay for the Rapid and Sensitive Detection of Ampicillin in Hospital Wastewater.
Honggui LinFeixiang FangJiahui ZangJianlong SuQingyuan TianRanjith Kumar KankalaXuexia LinPublished in: Micromachines (2020)
In this study, a convenient assay method has been developed based on labeled functional nucleic acids (H-DNA) and a competitive fluorescent lateral flow immunoassay (CF-LFI) for ampicillin (AMP) detection. Herein, we designed the tunable AMP probes for AMP detection based on the AMP aptamer, and the secondary DNA fragment. The probes can generate tunable signals on the test line (T line) and control line (C line) according to the concentration of AMP. The accuracy of detection was improved by optimizing the tunable AMP probes. Under the optimal conditions, the linear concentration of AMP detection is ranged from 10 to 200 ng/L with a limit of quantitation (LOQ) value of 2.71 ng/L, and the recovery is higher than 80.5 %. Moreover, the developed method shows the potential application for AMP detection in the hospital wastewater.
Keyphrases
- protein kinase
- loop mediated isothermal amplification
- sensitive detection
- label free
- quantum dots
- living cells
- single molecule
- small molecule
- real time pcr
- healthcare
- emergency department
- mass spectrometry
- wastewater treatment
- cell free
- gold nanoparticles
- climate change
- fluorescent probe
- high throughput
- adverse drug
- electronic health record