A novel and ultrasensitive electrochemical DNA biosensor for pralatrexate detection.
Leila GhasemiShohreh JahaniMahdiyeh GhazizadehMohammad Mehdi ForoughiPublished in: Analytical methods : advancing methods and applications (2023)
An attempt was made to develop a new sensitive biosensor for pralatrexate, as an anticancer drug, based on its interaction with the guanine of fish sperm DNA anchored on a screen-printed electrode (SPE) modified with polypyrrole (PP)/octahedral Pd-doped Co 3 O 4 composite (Oh-Pd-doped Co 3 O 4 C). Electrochemical techniques like differential pulse voltammetry verified the mechanism of such an interaction on the dsDNA/PP/Oh-Pd-doped Co 3 O 4 C/SPE surface. A reduction in the peak current of guanine oxidation elucidated the interaction in acetate buffer with pH = 4.8. The optimization of response was performed for the interaction method according to potential, accumulation time, reproducibility and drug content. The linear dynamic range was estimated at 1.0 nM to 150.0 μM as well as a limit of detection as low as 0.61 nM for the DNA and pralatrexate concentrations. The practical potential of the proposed sensor was verified by determining pralatrexate in its pharmaceutical matrices.
Keyphrases
- label free
- quantum dots
- gold nanoparticles
- circulating tumor
- molecularly imprinted
- cell free
- ms ms
- sensitive detection
- highly efficient
- visible light
- photodynamic therapy
- solid phase extraction
- loop mediated isothermal amplification
- blood pressure
- ionic liquid
- nucleic acid
- emergency department
- hydrogen peroxide
- nitric oxide
- tandem mass spectrometry
- carbon nanotubes
- neural network