COVID-19 measures implied many changes to travel behaviour and transport mode choice during the pandemic. This study seeks to understand what individual characteristics and travel attributes are related to transport mode choice before, during, and after the first lockdown in Italy. Based on an online survey (carried out in May 2020 in Milan), three multinomial regression models are presented. The results show that and in which measure parameters regarding distance and duration of daily travel are markedly related to transport mode before the lockdown. However, these factors are less significantly associated with the transport mode during and after the lockdown. Meanwhile, factors such as Preferences and Worry about using public transport have more significant relationship with the modal choice during the pandemic. Regarding individual characteristics, women are more likely to use active mode during and after the lockdown. Additionally, two personality traits of Agreeableness , and Openness to experiences are related to transport mode during and after the lockdown, respectively. Overall, this study reveals that in addition to socio-demographic factors, other variables such as worry about using public transport, preference, and personality are associated with the choice of transport mode during the lockdown.