A Novel Fluorescent Probe for the Detection of Hydrogen Peroxide.
Kangkang WangTingting YaoJiayu XueYanqiu GuoXiao-Wei XuPublished in: Biosensors (2023)
Hydrogen peroxide (H 2 O 2 ) is one of the important reactive oxygen species (ROS), which is closely related to many pathological and physiological processes in living organisms. Excessive H 2 O 2 can lead to cancer, diabetes, cardiovascular diseases, and other diseases, so it is necessary to detect H 2 O 2 in living cells. Since this work designed a novel fluorescent probe to detect the concentration of H 2 O 2 , the H 2 O 2 reaction group arylboric acid was attached to the fluorescein 3-Acetyl-7-hydroxycoumarin as a specific recognition group for the selective detection of hydrogen peroxide. The experimental results show that the probe can effectively detect H 2 O 2 with high selectivity and measure cellular ROS levels. Therefore, this novel fluorescent probe provides a potential monitoring tool for a variety of diseases caused by H 2 O 2 excess.
Keyphrases
- hydrogen peroxide
- fluorescent probe
- living cells
- reactive oxygen species
- cardiovascular disease
- nitric oxide
- cell death
- loop mediated isothermal amplification
- dna damage
- real time pcr
- type diabetes
- label free
- risk assessment
- squamous cell carcinoma
- skeletal muscle
- metabolic syndrome
- weight gain
- insulin resistance
- climate change
- young adults
- glycemic control
- quantum dots
- sensitive detection