A molecular chemodosimeter to probe "closed shell" ions in kidney cells.
Amine AsselMeagan M StanleyRashid MiaBesma BoulilaPeter J CraggIyanuoluwani OwolabiMeredith HetrickMosharrof MondalKarl J WallaceHichem Ben JannetPublished in: Organic & biomolecular chemistry (2023)
Two quinidine-functionalized coumarin molecular probes have been synthesized and have been found to bind metal cations (Cd 2+ , Co 2+ , Cu 2+ , Fe 2+ , Hg 2+ , Ni 2+ , and Zn 2+ ) with high affinity in organic-aqueous media (DMSO-HEPES). The chemodosimeters coordinate with the Zn 2+ ions in a two-to-one ratio (molecular probe : Zn 2+ ) with a log β of 10.0 M -2 . Upon the addition of the closed-shell metal ions studied, a fluorescence turn-on via an excimer formation is seen at 542 nm due to the quinaldine moiety adopting a syn arrangement when coordinated to the metal Zn 2+ ions. Confocal microscopy monitored free Zn 2+ ions in the Human Embryonic Kidney cell line HEK293 by coordinating with the chemodosimter.
Keyphrases
- quantum dots
- aqueous solution
- living cells
- heavy metals
- single molecule
- fluorescent probe
- sensitive detection
- water soluble
- endothelial cells
- induced apoptosis
- energy transfer
- signaling pathway
- photodynamic therapy
- oxidative stress
- cell death
- mass spectrometry
- high resolution
- endoplasmic reticulum stress
- molecularly imprinted
- pi k akt
- visible light