Microsatellite Instability Detection in Cancer: A Multiplex qPCR Approach that Obviates the Need for Matching Normal Samples.
Wei ChenYan Helen YanBlake YoungAlessandro PintoQi JiangNanjia SongAdam YaseenWeijie YaoDavid Yu ZhangJinny Xuemeng ZhangPublished in: Clinical chemistry (2024)
This study validates VarTrace as a swift, accurate, and economical assay for MSI detection in samples with low tumor percentages without the need for matching normal DNA. VarTrace's capacity for highly sensitive MSI analysis holds potential for enhancing the efficiency of clinical work flows and broadening the availability of this test.
Keyphrases
- real time pcr
- label free
- high throughput
- loop mediated isothermal amplification
- papillary thyroid
- circulating tumor
- single molecule
- high resolution
- squamous cell
- cell free
- human health
- young adults
- mass spectrometry
- fluorescent probe
- climate change
- molecularly imprinted
- lymph node metastasis
- childhood cancer
- nucleic acid
- sensitive detection
- liquid chromatography
- simultaneous determination