Using recombinase-mediated cassette exchange to engineer MIN6 insulin-secreting cells based on a newly identified safe harbor locus.

Asami FurukawaAya TanakaSuguru YamaguchiMinami KosudaMidori YamanaAkiko NagasawaGenta KohnoHisamitsu Ishihara

Published in: Journal of diabetes investigation (2021)

We engineered MIN6 cells, which can serve as a powerful tool for testing genetic alterations associated with diabetes, and studied the molecular mechanisms of insulin secretion.

Keyphrases

induced apoptosis
type diabetes
cell cycle arrest
cardiovascular disease
endoplasmic reticulum stress
oxidative stress
metabolic syndrome
adipose tissue
genome wide
skeletal muscle
weight loss