Purification and enzymatic characterization of the RNA ligase RTCB from Thermus thermophilus.
Shuyan DuanZijun ChenZhengyang LiRui JiJianhua GanJixi LiPublished in: Biotechnology letters (2019)
D64 was proved to be essential for RTCB-catalyzed RNA ligation and DNA activation (from 37 to 70 °C) in T. thermophilus.