Antitumor Effects of an Anthocyanin-Rich Grain Diet in a Mouse Model of Lewis Lung Carcinoma.
Maria A TikhonovaOlesya Yu ShoevaMichael V TenditnikAnna A AkopyanEkaterina A LitvinovaNelly A PopovaTamara G AmstislavskayaElena K KhlestkinaPublished in: International journal of molecular sciences (2024)
Functional foods enriched with plant polyphenol anthocyanins attract particular attention due to their health-promoting properties, including antitumor activity. We evaluated the effects of a grain diet rich in anthocyanins in a mouse model of Lewis lung carcinoma. Mice of the C57BL/6 strain were fed with wheat of near-isogenic lines differing in the anthocyanin content for four months prior to tumor transplantation. Although a significant decrease in the size of the tumor and the number of metastases in the lungs was revealed in the groups with both types of grain diet, the highest percentage of animals without metastases and with attenuated cell proliferation in the primary tumor were observed in the mice with the anthocyanin-rich diet. Both grain diets reduced the body weight gain and spleen weight index. The antitumor effects of the grain diets were associated with the activation of different mechanisms: immune response of the allergic type with augmented interleukin(IL)-9 and eotaxin serum levels in mice fed with control grain vs. inhibition of the IL-6/LIF system accompanied by a decrease in the tumor-associated M2 macrophage marker arginase 1 gene mRNA levels and enhanced autophagy in the tumor evaluated by the mRNA levels of Beclin 1 gene. Thus, anthocyanin-rich wheat is suggested as a promising source of functional nutrition with confirmed in vivo antitumor activity.
Keyphrases
- weight loss
- physical activity
- weight gain
- mouse model
- immune response
- cell proliferation
- body mass index
- high fat diet induced
- healthcare
- public health
- copy number
- type diabetes
- cell death
- gene expression
- working memory
- mental health
- birth weight
- genome wide
- endoplasmic reticulum stress
- single cell
- bone marrow
- mesenchymal stem cells
- climate change
- dna methylation
- risk assessment
- genome wide identification