High-resolution Inference of Multiplexed Anti-HIV Gene Editing using Single-Cell Targeted DNA Sequencing.
Mohamed S BouzidiZain Y DossaniCarolina Di BenedettoKyle A RaymondShivani DesaiLeonard R ChavezPaola BetancurSatish K PillaiPublished in: bioRxiv : the preprint server for biology (2024)
Bouzidi et al created a novel targeted single-cell DNA sequencing pipeline to characterize anti-HIV gene editing at multiple viral and host genome sites within individual cells, providing the HIV cure field with a powerful tool to gauge the preclinical and clinical efficacy of HIV gene therapy approaches.
Keyphrases
- single cell
- antiretroviral therapy
- hiv positive
- hiv testing
- hiv infected
- human immunodeficiency virus
- rna seq
- hepatitis c virus
- hiv aids
- men who have sex with men
- high resolution
- gene therapy
- high throughput
- induced apoptosis
- south africa
- single molecule
- circulating tumor
- sars cov
- stem cells
- cancer therapy
- mesenchymal stem cells
- drug delivery
- cell proliferation
- gene expression
- signaling pathway
- genome wide
- cell therapy