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Translocation of LSR from tricellular corners causes macropinocytosis at cell-cell interface as a trigger for breaking out of contact inhibition.

Takayuki KohnoTakumi KonnoShin KikuchiMasuo KondohTakashi Kojima
Published in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2021)
Withdrawal from contact inhibition is necessary for epithelial cancer precursor cells to initiate cell growth and motility. Nevertheless, little is understood about the mechanism for the sudden initiation of cell growth under static conditions. We focused on cellular junctions as one region where breaking out of contact inhibition occurs. In well-differentiated endometrial cancer cells, Sawano, the ligand administration for tricellular tight junction protein LSR, which transiently decreased the robust junction property, caused an abrupt increase in cell motility and consequent excessive multilayered cell growth despite being under contact inhibition conditions. We observed that macropinocytosis essentially and temporarily occurred as an antecedent event for the above process at intercellular junctions without disruption of the junction apparatus but not at the apical plasma membrane. Collectively, we concluded that the formation of macropinocytosis, which is derived from tight junction-mediated signaling, was triggered for the initiation of cell growth in static precancerous epithelium.
Keyphrases
  • single cell
  • cell therapy
  • blood brain barrier
  • single molecule
  • induced apoptosis
  • stem cells
  • mesenchymal stem cells
  • young adults
  • cell proliferation
  • amino acid
  • endometrial cancer
  • cell cycle arrest